plv ttr krab red Search Results


kras  (Sino Biological)
93
Sino Biological kras
Kras, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plv ttr krab ires red  (Addgene inc)
93
Addgene inc plv ttr krab ires red
Plv Ttr Krab Ires Red, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bicistronic vector called plv ttr krab zeo expressed tet krab  (InvivoGen)
86
InvivoGen bicistronic vector called plv ttr krab zeo expressed tet krab
Bicistronic Vector Called Plv Ttr Krab Zeo Expressed Tet Krab, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plv-ut-ttr-krab lentiviral vector  (Addgene inc)
90
Addgene inc plv-ut-ttr-krab lentiviral vector
Plv Ut Ttr Krab Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plv ttr krab red  (Addgene inc)
92
Addgene inc plv ttr krab red
Plv Ttr Krab Red, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plv ttr krab red - by Bioz Stars, 2026-04
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ef1 dcas9 kras  (Addgene inc)
93
Addgene inc ef1 dcas9 kras
( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible <t>dCas9-DD-BirA,</t> and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.
Ef1 Dcas9 Kras, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ef1 dcas9 kras - by Bioz Stars, 2026-04
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zfn-krab  (Biomol GmbH)
90
Biomol GmbH zfn-krab
( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible <t>dCas9-DD-BirA,</t> and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.
Zfn Krab, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gene set krab  (InterPro Inc)
90
InterPro Inc gene set krab
( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible <t>dCas9-DD-BirA,</t> and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.
Gene Set Krab, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet28 mhl krasb  (Addgene inc)
93
Addgene inc pet28 mhl krasb
( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible <t>dCas9-DD-BirA,</t> and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.
Pet28 Mhl Krasb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pet28 mhl krasb - by Bioz Stars, 2026-04
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krab domain  (Addgene inc)
90
Addgene inc krab domain
( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible <t>dCas9-DD-BirA,</t> and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.
Krab Domain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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krab-mecp2  (Addgene inc)
90
Addgene inc krab-mecp2
( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible <t>dCas9-DD-BirA,</t> and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.
Krab Mecp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pet28-mhl krasb  (Addgene inc)
90
Addgene inc plasmid pet28-mhl krasb
( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible <t>dCas9-DD-BirA,</t> and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.
Plasmid Pet28 Mhl Krasb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible dCas9-DD-BirA, and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.

Journal: bioRxiv

Article Title: Active and repressed chromatin domains exhibit distinct nucleosome segregation during DNA replication

doi: 10.1101/418707

Figure Lengend Snippet: ( A ) Overview of the system to assess in vivo chromatin domain inheritance in mESCs. A master cell line containing endogenous tags of Flag-BAP H3.1 and -H3.2, stable integration of doxycycline (Dox)-inducible dCas9-DD-BirA, and transducible gRNAs spanning 5 kb of a candidate locus is arrested in G1. Following a pulse of doxycycline (Dox) and exogenous biotin, nearby tagged parental nucleosomes are biotinylated (blue histones and yellow asterisks). Wash-off of media releases cells into S-phase wherein the re-distribution of biotin-H3 at a mononucleosomal level is assayed in newly synthesized chromatin. ( B ) Native Flag and biotin ChIP-seq analysis of G1/S-blocked cells at the HoxC cluster following dCas9-DD-BirA recruitment. ( C ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs showing biotin enrichment at the Hoxc6 locus compared to Ebf1 , Meis2 , Ccna2 , Gapdh and IgG controls. ( D ) Flag and biotin native ChIP-seq analysis of cells at the Ebf1 locus following dCas9-DD-BirA recruitment. ( E ) Native ChIP-qPCR analysis of biotin-H3 in G1/S-blocked mESCs, validating biotin enrichment at the Ebf1 locus compared to Hoxc6 , Meis2 , Ccna2 , Gapdh and IgG controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.

Article Snippet: The cDNA for dCas9 was initially cloned from EF1-dCas9-KRAS (pHAGE EF1α dCas9-KRAB was a gift from Rene Maehr and Scot Wolfe, Addgene plasmid #50919) ( ) into pINTO-C-HF (pcDNA4/TO from Invitrogen with a C-terminus HA-Flag tag) using EcoR1 and Not1.

Techniques: In Vivo, Synthesized, ChIP-sequencing

( A ) Cell cycle analysis corresponding to G1/S-blocked and released mESCs at time 0 hr -1 cell, 12 hr -2 cell, 24 hr - 4 cells, and 48 hr - 16 cells. ( B ) Cas9 ChIP-qPCR analysis of G1/S-blocked and released mESCs following a 6 hr pulse with minimal doxycycline (Dox) and exogenous biotin in cells targeting dCas9-DD-BirA to the Hoxc6 locus compared to Gapdh controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.

Journal: bioRxiv

Article Title: Active and repressed chromatin domains exhibit distinct nucleosome segregation during DNA replication

doi: 10.1101/418707

Figure Lengend Snippet: ( A ) Cell cycle analysis corresponding to G1/S-blocked and released mESCs at time 0 hr -1 cell, 12 hr -2 cell, 24 hr - 4 cells, and 48 hr - 16 cells. ( B ) Cas9 ChIP-qPCR analysis of G1/S-blocked and released mESCs following a 6 hr pulse with minimal doxycycline (Dox) and exogenous biotin in cells targeting dCas9-DD-BirA to the Hoxc6 locus compared to Gapdh controls. Data was normalized to 5% input and error bars represent standard error of three biological replicates.

Article Snippet: The cDNA for dCas9 was initially cloned from EF1-dCas9-KRAS (pHAGE EF1α dCas9-KRAB was a gift from Rene Maehr and Scot Wolfe, Addgene plasmid #50919) ( ) into pINTO-C-HF (pcDNA4/TO from Invitrogen with a C-terminus HA-Flag tag) using EcoR1 and Not1.

Techniques: Cell Cycle Assay